Addgene 73178

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The Brunello guide libr ary (Addgene #73178), which targ ets 19, 114 human genes with 4 guides each 13 was . 31. packaged in HEK 293T cells as descri bed above. 32. 33. 1.2.2 Generatio n of Cas9 ...gabay jacayl oo maskaxaysan, Soomaalida waa dad caan ku ah xagga gabayada iyo noocyada kala duwan ee suugaanta, balse dadka qaar ayaa wax dheer kuwa kale marka laga hadlayo arrintan.

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library (Addgene #73178) containing 77,440 sgRNAs targeting 19,110 genes (12, 13), HEK293T cells were seeded at 40% confluence in T225 flasks. After 24 hours, medium was replaced with 13 mL OptiMEM 1 hour before transfection with plasmids encoding the library. 20 mg of Brunello plasmid, 10 mg of pMD2.G, and 15 mgof
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Nov 13, 2019 · Three ATLL cell lines were transduced with lentiviral construct for Cas9 nuclease, followed by lentiviral delivery of the human CRISPR Brunello pooled library (Addgene 73178) of 76,456 single-guide RNAs targeting 19,144 protein-coding genes to cause DNA double-stranded cleavage by the Cas9 nuclease and loss-of-function of the respective genes.
Human CRISPR Knockout Pooled Library (Brunello) pooled lentivirus 73178-LV ... Addgene. 490 Arsenal Way Ste 100 Usa 02472-2870. Line Items. Line # Description Qty
Sep 23, 2020 · Human CRISPR knockout pooled library (Brunello) was obtained from Addgene (#73178). Human CRISPRi pooled library (Dolcetto) was a gift from John Doench (Broad Institute, also available on Addgene #92385).
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Jan 16, 2020 · David Root and John Doench, Addgene: 73178-LV: Plasmids: Mouse-GPR161-FLAG: Insight Biotechnology LTD: MR224248: Human-TMEM220-FLAG: Genescript USA INC: OHu14539: Human-COL14A1-FLAG: Stratech Scientific LTD: HG13622-CF-SIB: Mouse-FAM98B-FLAG: Insight Biotechnology LTD: MR206836: Mouse-FAM102B-FLAG: Insight Biotechnology LTD: MR212923: Mouse-MXRA7-FLAG: Insight Biotechnology LTD: MR218215
To generate the lentiviral particles for the Brunello whole genome library (Addgene #73178) containing 77,440 sgRNAs targeting 19,110 genes, (12,13), HEK293T cells were seeded at 40 % confluence in T225 flasks. After 24 h, medium was replaced with 13 ml OptiMEM 1 h before
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David Root and John Doench, Addgene: 73178-LV: Plasmids: Mouse-GPR161-FLAG: Insight Biotechnology LTD: MR224248: Human-TMEM220-FLAG: Genescript USA INC: OHu14539: Human-COL14A1-FLAG: Stratech Scientific LTD: HG13622-CF-SIB: Mouse-FAM98B-FLAG: Insight Biotechnology LTD: MR206836: Mouse-FAM102B-FLAG: Insight Biotechnology LTD: MR212923: Mouse ...
Brunello in lentiGuide-Puro) transfer vector (Addgene, cat. #73178) [17], 16.7μg of psPAX2 plasmid (Addgene, cat. # 12260), and 11μg of pMD2.G plasmid (Addgene, cat. # 12259) were combined with Lipofectamine 3000 (Thermo Fisher Scientific) in accordance with the manu-facturer's protocol, and the mixture was used to trans-fect the cells.
addgene.org reaches roughly 28,974 users per day and delivers about 869,230 users each month. The domain addgene.org uses a Commercial suffix and it's server(s) are located in US with the IP number 172.67.23.232 and it is a .org domain.
Sorted cells were subsequently transduced with a pLenti-CMV TetR Blast (Addgene) and selected with blasticidin (7μg/ml). pLenti CMV TetR Blast (716-1) was a gift from Dr. Eric Campeau (Addgene plasmid #17492) ( ). .. All expression experiments were performed on day 2 of doxycycline (1 μg/ml) induction.
Optimalizované metriky pre obrazovky CRISPR-KO s knižnicami gRNA druhej generácie
Optimalizované metriky pre obrazovky CRISPR-KO s knižnicami gRNA druhej generácie
Brunello in lentiGuide-Puro) transfer vector (Addgene, cat. #73178) [17], 16.7μg of psPAX2 plasmid (Addgene, cat. # 12260), and 11μg of pMD2.G plasmid (Addgene, cat. # 12259) were combined with Lipofectamine 3000 (Thermo Fisher Scientific) in accordance with the manu-facturer’s protocol, and the mixture was used to trans-fect the cells.
73178: 3.3: CEBPG CREB1 TARDBP ZHX2 FOXA1 SP1 ATF7 BMI1 ESR1 CTBP1: LINC01876 NR4A2 piR-42694-088: GH02J156319: Promoter/Enhancer: 1.6: Ensembl ENCODE CraniofacialAtlas dbSUPER: 5.1-65.7-65651: 2: CTCF RFX1 POLR2A ZNF740 EZH2 MGA CBX3 UBTF EGR1 MNT: ENSG00000282440 NR4A2 LINC01876 lnc-GPD2-1
Optimalizované metriky pre obrazovky CRISPR-KO s knižnicami gRNA druhej generácie

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To generate the lentiviral particles for the Brunello whole genome library (Addgene #73178) containing 77,440 sgRNAs targeting 19,110 genes, (12,13), HEK293T cells were seeded at 40 % confluence in T225 flasks. After 24 h, medium was replaced with 13 ml OptiMEM 1 h before
73178: 3.3: CEBPG CREB1 TARDBP ZHX2 FOXA1 SP1 ATF7 BMI1 ESR1 CTBP1: LINC01876 NR4A2 piR-42694-088: GH02J156319: Promoter/Enhancer: 1.6: Ensembl ENCODE CraniofacialAtlas dbSUPER: 5.1-65.7-65651: 2: CTCF RFX1 POLR2A ZNF740 EZH2 MGA CBX3 UBTF EGR1 MNT: ENSG00000282440 NR4A2 LINC01876 lnc-GPD2-1
National Center for Biotechnology Information
Nov 13, 2019 · Three ATLL cell lines were transduced with lentiviral construct for Cas9 nuclease, followed by lentiviral delivery of the human CRISPR Brunello pooled library (Addgene 73178) of 76,456 single-guide RNAs targeting 19,144 protein-coding genes to cause DNA double-stranded cleavage by the Cas9 nuclease and loss-of-function of the respective genes.
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This is the command I ran: python count_spacers.py -f BSF_0622_H72K5BBXY_1_Brun_73178_S52986.fq -o library_count.csv -i Brunello_list.csv (without the no-g since I have the G appended at the 5' end of the guide sequence).
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Feb 25, 2020 · To generate the lentiviral particles for the Brunello whole genome library (Addgene #73178) containing 77,440 sgRNAs targeting 19,110 genes, (12,13), HEK293T cells were seeded at 40 % confluence in T225 flasks. After 24 h, medium was replaced with 13 ml OptiMEM 1 h before
Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues. Powered by Zendesk ...
Protocol: Amplification of pDNA libraries MATERIALS • 100 µL electrocompetent cells (STBL4TM, Thermo Fisher Scientific, 11635-018) • 400 ng library plasmid DNA • 4 electroporation cuvettes (0.1 cm gap, Bio-Rad, 165-2089)
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The production of a high-titer human sgRNA Brunello lentiviral library, which contains 4 sgRNAs per gene (no. 73178; Addgene), was performed by transfecting HEK293T cells in five 15-cm tissue culture plates using the polyethyleneimine (PEI) (linear; molecular weight [MW], 25,000; no. 23966-1-A; Polysciences) transfection method . Briefly, for ...
Jan 16, 2020 · David Root and John Doench, Addgene: 73178-LV: Plasmids: Mouse-GPR161-FLAG: Insight Biotechnology LTD: MR224248: Human-TMEM220-FLAG: Genescript USA INC: OHu14539: Human-COL14A1-FLAG: Stratech Scientific LTD: HG13622-CF-SIB: Mouse-FAM98B-FLAG: Insight Biotechnology LTD: MR206836: Mouse-FAM102B-FLAG: Insight Biotechnology LTD: MR212923: Mouse-MXRA7-FLAG: Insight Biotechnology LTD: MR218215
The production of a high-titer human sgRNA Brunello lentiviral library, which contains 4 sgRNAs per gene (no. 73178; Addgene), was performed by transfecting HEK293T cells in five 15-cm tissue culture plates using the polyethyleneimine (PEI) (linear; molecular weight [MW], 25,000; no. 23966-1-A; Polysciences) transfection method . Briefly, for ...



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